| Tissue chambers and microscope stage adapters | ||||
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| The BT-1xxx tissue chambers all have inflow and outflow via a thin slit to promote laminar flow for flow rates up to 2ml/min. Typical flow rates for the BT-1, BT-1AR are 1ml/min. In applications using an inverted microscope with air type objective and upright microscope you can use a transparent ITO heater (eg. HI-24p, HI-25Dp) to heat the chamber directly from below. If perfusing, the inflow is heated with the HPRE2 Pre-heater which connects to the inlet tube. The chambers have a thermistor groove to allow permanent placement of the thermistor sensor for measurement/control of the chamber temperature. With upright microscopes you could also heat the objective with an objective heater (eg. HLS-1p) For inverted microscopes using immersion lenses there is typically insufficient working distance to use an ITO heater so you use a thin cover slide for the bottom of the chamber and an objective heater. At flow rates >1ml/min most heating is done by the pre-heater. Heated metal stages provide very little heat transfer to chambers. For cell culture applications consider our Reusable culture chamber system (CSTPKG). |
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