| Tissue chambers and microscope stage adapters | ||||
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| The BT-1xxx tissue chambers all have inflow and outflow via a thin slit to promote laminar flow for flow rates up to 2ml/min. Typical flow rates for the BT-1, BT-1AR are 1ml/min. In applications using an inverted microscope with air type objective and upright microscope you can use a transparent ITO heater (eg. HI-24p, HI-25Dp) to heat the chamber directly from below. If perfusing, the inflow is heated with the HPRE2 Pre-heater which connects to the inlet tube. The chambers have a thermistor groove to allow permanent placement of the thermistor sensor for measurement/control of the chamber temperature. With upright microscopes you could also heat the objective with an objective heater (eg. HLS-1p) For inverted microscopes using immersion lenses there is typically insufficient working distance to use an ITO heater so you use a thin cover slide for the bottom of the chamber and an objective heater. At flow rates >1ml/min most heating is done by the pre-heater. Heated metal stages provide very little heat transfer to chambers. We have also a new range of brain slice chambers suitable for the Siskiyou 8090C stage and for use with our own microscope stage adapters. For cell culture applications consider our Reusable culture chamber system (CSTPKG). |
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 Stage Adapters |
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